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B vitamin (B9)

Folate, the cell builder

Folate is a B vitamin that helps make DNA and new cells1, and folic acid, its form in supplements and fortified foods, helps prevent neural tube defects3.

VitaminsTracked in mcgDaily target3 sources · checked 2026-09-27

How much you need

Close-up of fresh green asparagus tips standing upright against a soft green background
Photo: engin akyurt on Unsplash (Unsplash License)
01 What it does

What folate does for you.

DNA and cell division

Folate is needed to make DNA and RNA and for proper cell division1.

Early pregnancy

Folic acid before and during early pregnancy helps prevent neural tube defects3.

Red blood cells

Folate helps form red blood cells, and low folate can cause megaloblastic anaemia2.

Protein building

Folate works with vitamin B12 and vitamin C to break down, use and make proteins2.

02 How your body uses it

How your body uses folate.

How your body uses folate: Food folate → Gut → Absorption → Activation → Bloodstream → Cells. Folic acid skips the split step Absorption.01Food folateGreens, beans, liver02GutSplit to monoglutamate03AbsorptionAcross the gut lining04ActivationReduced to THF forms05BloodstreamMainly as 5-MTHF06CellsDNA, RNA, cell divisionFolic acidFortified foods, supplementsskips the split stepHow your body uses folate: Food folate → Gut → Absorption → Activation → Bloodstream → Cells. Folic acid skips the split step Absorption.01Food folateGreens, beans, liver02GutSplit to monoglutamate03AbsorptionAcross the gut liningFolic acidFortifiedfoods,supplements04ActivationReduced to THF forms05BloodstreamMainly as 5-MTHF06CellsDNA, RNA, cell division

Food folates are split into a single-glutamate form in the gut, absorbed, then converted so that 5-MTHF is the main form in blood1. Folic acid is already in that single-glutamate form1.

03 Where to get it

Foods and other sources.

AsparagusBlack-eyed peasBrussels sproutsRomaine lettuceAvocadoBroccoliKidney beansOrangesEnriched bread

Vegetarian

Food and servingAmount
Spinach, boiled, ½ cup131 µg DFE
Black-eyed peas, boiled, ½ cup105 µg DFE
Asparagus, boiled, 4 spears89 µg DFE
Brussels sprouts, boiled, ½ cup78 µg DFE
Romaine lettuce, shredded, 1 cup64 µg DFE
Avocado, sliced, ½ cup59 µg DFE
Broccoli, cooked, ½ cup52 µg DFE
Kidney beans, canned, ½ cup46 µg DFE

Non-vegetarian

Food and servingAmount
Beef liver, braised, 3 oz215 µg DFE
Crab, Dungeness, 3 oz36 µg DFE

Amounts are in µg DFE, a unit that accounts for folic acid being absorbed better than natural food folate1. Amounts per serving from1.

Enriched grains

In the U.S., enriched breads, cereals, flours, pasta and rice have added folic acid1.

Supplements

Folic acid is in multivitamins, prenatal vitamins and B-complex products, and some supplements use 5-MTHF1.

Gut bacteria

Bacteria in the colon make some folate, though how much this adds to folate status is unclear1.

04 How much you need

Your daily reference amounts.

The same reference table draws the reference lines on your charts in Ayuvo. Needs differ in pregnancy and breastfeeding, which are not covered here.

Folate: daily RDA by age and sex
AgeWomenMenUpper limit
19–30400 mcg400 mcg1,000 mcg
31–50400 mcg400 mcg1,000 mcg
51–70400 mcg400 mcg1,000 mcg
71+400 mcg400 mcg1,000 mcg

RDA (Recommended Dietary Allowance) is the daily amount that meets the needs of nearly all healthy people. Per day, for adults1.

Upper limit (UL) is the most per day that is unlikely to cause harm. Applies to folic acid from supplements and fortified foods (mcg, not mcg DFE), not folate naturally in food.

  • Amounts are in mcg dietary folate equivalents (DFE); 1 mcg DFE = 0.6 mcg folic acid taken with food.
Folate reference amounts for adults aged 31–50: RDA 400 mcgRDA 400 mcg0Adults 31–50, per day
Below the recommended amountMeets the reference

The upper limit for folate covers only part of your intake (for folic acid from fortified foods and supplements only, not natural food folate), so it is not drawn on this bar.

05 Balance

Too little, too much.

Signs like these have many possible causes. They are a reason to talk to a clinician, not to self-treat.

Too little

Low folate can cause megaloblastic anaemia and usually occurs alongside other nutrient shortfalls1.

  • Weakness and fatigue
  • Trouble concentrating
  • Irritability and headache
  • Heart palpitations
  • Shortness of breath
  • Mouth sores, sore tongue

Too much

Folate from food has not been linked with harm, so the upper limit on this page applies only to folic acid from supplements and fortified foods1.

  • May mask B12-related anaemia
  • B12 nerve damage can go unnoticed
  • Unmetabolised folic acid in blood
  • Other effects still being studied
06 Blood test

Serum and red cell folate.

Serum folate reflects recent intake, while red blood cell folate gives a longer-term view of folate status1.

ResultWhat it may mean
Serum folate above 3 ng/mLAdequate (reflects recent intake)
Red cell folate above 140 ng/mLAdequate longer-term status

Labs use different units and ranges. Your clinician reads a result alongside your history and other tests.

07 Supplements

Supplement forms.

At least 85% of folic acid taken with food is absorbed, compared with about 50% of the folate naturally in food1.

Folic acid

The form in most supplements and fortified foods, and the only form shown to help prevent neural tube defects3.

5-MTHF (methylfolate)

Absorbed as well as or better than folic acid; its conversion to DFE is not formally set1.

Supplements can interact with medicines. Talk to a clinician or pharmacist before you start one, especially at high doses.

08 Key points

Remember these five.

  1. Folate is the natural form in food; folic acid is the form in supplements and fortified foods.
  2. Neural tube defects form in the first weeks of pregnancy, often before it is known.
  3. Leafy greens, beans, asparagus and oranges all provide folate.
  4. The upper limit applies to folic acid only, not to folate naturally found in food.
  5. High folic acid can hide the anaemia of low B12, so B12 status still matters.
FAQ

Questions about folate.

What is the difference between folate and folic acid?

Folate is the general name for all forms of vitamin B9, including those found naturally in food. Folic acid is the form used in supplements and fortified foods, and the body absorbs it better than food folate.

Why is folic acid important before pregnancy?

Neural tube defects such as spina bifida happen in the first few weeks of pregnancy, often before someone knows they are pregnant. Getting enough folic acid before and during early pregnancy helps prevent them.

Can you get too much folate from food?

High intakes of folate from food have not been linked with harm. The upper limit applies only to folic acid from supplements and fortified foods, partly because large amounts can mask low vitamin B12.

Get started

Track folate in Ayuvo.

Ayuvo charts folate by day, week, month and year, with the reference lines from this page, personalised by age and sex where they differ. Supplements you log in Medications count too. Free, with no account.

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Sources

Facts on this page come from these sources, checked on the dates shown. Intake numbers come from Ayuvo's shared reference table, which cites the sources marked for them.

  1. Folate: Fact Sheet for Health Professionals · NIH Office of Dietary Supplements checked 2026-09-27
  2. Folic acid in diet · MedlinePlus, U.S. National Library of Medicine checked 2026-09-27
  3. About Folic Acid · Centers for Disease Control and Prevention checked 2026-09-27

General information, not medical advice. This guide is for healthy adults and does not cover pregnancy, breastfeeding, children or medical conditions. It cannot diagnose or treat anything. Talk to a clinician before starting high-dose supplements, or if you think you may have too little or too much of a nutrient. Spotted an error? Email us.